THE METABOLISM OF d-GALACTOSE IN PSEUDOMONAS SACCHAROPHILA

The metabolism of D-galactose in Pseudomonas saccharophila.

The amylases of pseudomonas saccharophila.

In his original study of Pseudomonas saccharophila, Doudoroff (1940) found that an inducible extracellular amylase was formed when cells were grown with soluble starch or maltose. He also observed indications of intracellular amylase activity. The present study was undertaken in order to characterize further the amylase(s) of P. saccharophila. Two enzymes with different physical and enzymatic p...

Phosphoribokinase from Pseudomonas saccharophila.

1. An enzyme catalyzing the formation of ribose 1,5-diphosphate from ribose 5-phosphate and ATP has been purified 40-fold from sonic extracts of Pseudomonas saccharophila. This preparation is free of both phosphoribomutase and phosphoglucomutase activity. It is specific for ribose S-phosphate as substrate; ribose l-phosphate, glucose 6-phosphate, and glucose l-phosphate fail to react. 2. The ap...

Sulfur incorporation into the alpha-amylase of Pseudomonas saccharophila.

Studies by Rotman and Spiegelman (1954) and Hogness et al. (1955) on the 3-galactosidase of Escherichia coli have revealed that virtually all of the carbon and sulfur of that enzyme is derived from exogenous sources when cells are induced during growth. These investigations have thus ruled out the direct participation of a specific preformed precursor in the formation of fl-galactosidase in gro...

The oxidation of L-arabinose by Pseudomonas saccharophila.

The importance of the pentoses in the physiology of plants and animals has been known since ribose and deoxyribose were identified as constituents of nucleic acids. Interest in pentose metabolism has been stimulated in the past few years by the discovery that ribose-5-phosphate and ribulosed-phosphate were integrant parts of the oxidative breakdown of glucose (1). The subject of pentose and pen...